Abstract: In the presented research, lipase production was carried out using consortium of Aspergillus niger and Trichoderma viride (simultaneously) in shake flasks. The effect of three factors on the production rate of lipase was studied. The selected three factors were including different concentrations of rapeseed oil (1.0 and 1.5%), pH (5.0 and 6.0) and incubation temperature (25 and 30°C). These factors and their optimized levels were analyzed by using 23 level factorial designs. Rapeseed oil concentration and pH were found to have positive effects on the production and secretion rate of lipase from consortium. However, the effect of pH was being larger than concentration of Rapeseed oil. Temperature was also effective but with minor importance than the other two factors. Results indicated that optimum conditions for the production of lipase from A. niger and T. viride consortium are 30°C with 1.0% Rapeseed oil as carbon source and medium pH 5.0.