Abstract: In this study, polysaccharide obtained from Neisseria meningitidis serogroup A was purified according to published protocol of World Health Organization. The outer Membrane Vesicle (OMV) of meningococcal serogroup B was also extracted by ultracentrifuge employing deoxycholate reagent. The derivatives of polysaccharide of meningococcal serogroup A (GAMP) was conjugated to OMV of meningococcal serogroup B which was treated by 1-ethyl-3- (3 dimethylaminopropyl) carbodiimide (EDAC).The obtained polysaccharide was activated by cyanogen bromide (CNBr) and 1-cyano-4-dimethy laminopyridinium tetrafluoroborate (CDAP) separately. First Adipic acid dihydrazide (ADH) with EDAC was bound to GAMP activated with CNBr to form GAMPCNBr AH. Second ADH with EDAC was bound to GAMP activated with CDAP to form GAMPCDAP AH. Then these derivatives were conjugated to OMV by EDAC to form GAMPCNBr AH-OMV and GAMPCDAP AH-OMV. The yields of GAMPCDAPAH-OMV was 45 to 48% which was higher than that of the other prepared conjugate (15-17%). The glycoconjugates were shown to induce hyper immunity in rabbits and formed antibodies against the above mentioned conjugates were detected by immunodiffusion technique.