Abstract: To investigate the antibiotic susceptibility, Vibrio parahaemolyticus were isolated and identified from the sample of shrimps collected from different shrimp processing plants located at Rupsha, Khulna. Among the 27 samples, 10 strains were identified as Vibrio parahaemolyticus. Susceptibility of these Vibrio parahaemolyticus was assayed by disc diffusion method against 11 antibiotics. All these strains were highly sensitive to Tetracycline, Norfloxacin, Gentamicin, Riphampicin and Neomycin, but were resistant to Erythromycin, Penicillin, Ampicillin and Kanamycin. Vibrio parahaemolyticus showed both resistant and susceptible character against Cephalexin and Streptomycin. 7 strains were resistant and 3 were sensitive to Cephalexin on the other hand 8 were resistant and 2 were sensitive to Streptomycin, among the 10 Vibrio parahaemolyticus strains. The antibiotics named Tetracycline, Norfloxacin, Gentamicin, Riphampicin and Neomycin should be the choice to control diseases due to Vibrio parahaemolyticus as well as enteric bacteria.