Abstract: In this study, various kinds of fruits and flowers obtained locally in Chiang Mai, Thailand were used as sources for acetic acid bacteria isolation. Using five enrichment media (with pH adjustment to 3.5), a total of one hundred and twenty-four strains were isolated. They were all Gram-negative, rod-shaped and produced clear zones on basal agar plates supplement with calcium carbonate. Of 124 strains, seventy-two (~58%) were derived from fruit samples. These bacterial strains were then characterised based on their cell morphology and biochemical assay; they can be classified in five Genera: Acetobacter (~14%), Acidomonas (~13%), Asaia (~31%), Gluconacetobacter (~17%) and Gluconobacter (25%).