Abstract: This study has designed and performed in the aim of cloning a specific toxoplasma antigen for further studies. We have amplified gene of P43 of toxoplasma tachyzoite and bradyzoite surface antigen. PCR product was cloned in pGEMEX-1 expression vector (named pGEM43) and is ready to make the recombinant protein for using as antigen. It can be used for either diagnosis or prevention of parasite in men.