Abstract: Remediation by Enhanced Natural Attenuation (RENA) was employed to remediate an oil contaminated site in the Gokana Local Government Area of Rivers State, Nigeria between January and September 2006. Mineral salt medium to which crude oil had been added was used as a sole source of carbon and energy to isolate hydrocarbon utilizers from the sample collected from different plots of the contaminated site. Two fungi, Articulosporium inflata and Zoopage mitospora; as well as five genera of bacteria, Lactobacter, Arthrobacter, Bacillus, Pseudomonas and Micrococcus were isolated and identified. The microbial and physicochemical properties of the soil samples varied with the different plots and at different periods of remediation.