Abstract: Some biochemical parameters during growth of Mucor rouxii and chitosan production in molasses-salt medium were determined. Growth of the organism started with an initial lag of 3 h; maximum growth and chitosan production were obtained at 32 h of fermentation. Fall in sugar concentration from 40.05 to 3.4 g L-1 was noted during the course of fermentation. Degree of acetylation of chitosan was more or less the same whereas increase in molecular weight was noted with the time of fermentation. Maximum 0.61 g chitosan of molecular weight 2.48 x 104 with ~13.0% acetylation could be harvested from mycelia of M. rouxii grown in 1 L of molasses salt medium.