Abstract: In the present study we have investigated the prevalence of tuberculosis and non tuberculosis Mycobacterium. Our results showed that 60 of 150 samples of sputum positive were cultured positive. The results showed that 66.7% (40/60) Mycobacterium tuberculosis, whereas 33.3% (20/60) were non tuberculosis mycobacterium. We also found that 3.33% (2/60) were Mycobacterium kansasii (MK) and 1.67% (1/60) Mycobacterium avium complex (MAC) and 28.33% (17/60) were other types. In conclusion, the percentage of Mycobacterium tuberculosis was significantly higher than that of the non tuberculosis Mycobacterium.