Abstract: Background and Objective: Proper knowledge on reproductive biology is important for the successful management of fisheries, aquaculture and conservation of a target species in the wild. The present study was investigated to uncover the reproductive biological information including the spawning season of Xenentodon cancila . Materials and Methods: About 325 Xenentodon cancila samples were collected from the waters of North-East Bangladesh for a period of 1 year. Data on length, weight, sex ratio, condition factor, fecundity and Gonado Somatic Index (GSI) were recorded to uncover the breeding biology of this fish in wild condition. Results: The length ranged between 20.20-23.91 cm (Mean±SD = 20.57±2.59) and their weight ranged between 19.70-43.85 g (Mean±SD = 30.22±8.12). Females were dominant in larger length groups being most apparent during the resting phase of reproductive cycle and in the peak of the spawning period, occurring in the month of June. The sexual ratio of all specimens was m/f = 1:1.11. The mean batch fecundity was 1789.69±347.06 of matured oocytes per ovary. Gonado somatic index data showed continuously increasing trend during the months of January-April until it reaches to the peak in June and followed by gradually decreasing in the months of September-November. Conclusion: On the basis of the collected data, it can be concluded that spawning season for this fish in wild ranged from April-August while peak in June in weather condition of North-East Bangladesh.