Abstract: Motile Aeromonad Septicaemia (MAS) disease in catfish (Clarias gariepinus) is caused by infection with Aeromonas hydrophila. One alternative way to control infection by this bacteria is applying probiotics, which inhibit quorum sensing, also known as Anti-Quorum Sensing (AQS), using Bacillus sp. administered by feeding. Bacillus sp. product AHL (Acyl Homoserine Lactone) Lactonase, which is speculated to be able to inhibit quorum sensing in pathogenic bacteria. In vivo test of probiotics was set up in triplicate and consisted of five treatments. Effect of AQS probiotic bacteria as feed supplement was set up in improving survival rate, Feed Conversion Ratio (FCR), Specific Growth Rate (SGR) and non specific immune response of catfish (Clarias gariepinus). The result showed that the fish feed with AQS had a high percentage of survival rate. The AQS mix of treatment (TS1RifR, TS2RifR and TA23RifR) had the highest survival rate (93%). The AQS treatments were also had values of FCR and SGR better than that of control. However, the control treatments had survival rate only 31%. The AQS isolates could also improve a non specific immune response. Result indicated that AHLs degrading bacteria could be considered as better alternative to replace application of antibiotics in aquaculture as biocontrol of bacterial fish disease and in reducing the pathogenicity of A. hydrophila in catfish (Clarias gariepinus).