Abstract: The proximate and fatty acid composition of oil extracted from catfish viscera (Clarias gariepinus) and red palm oil was carried out in this study, fish oil was extracted using wet rendering method. The result of the proximate composition shows moisture content of 64.81±2.37% for catfish viscera and 5.75±1.40% for red palm oil, protein content 16.40±2.66% for catfish viscera and 3.27±0.11% for red palm oil, ash content 120±0.20 and 1.04±0.30% for catfish viscera and palm oil, respectively lipid content 3.00±0.10 and 50.40±0.84%, Peroxide Value (PV) 16.60±0.40 and 1.0±0.42% and Iodine Value (IV) 140.05±0.14 and 51.07±0.47% for catfish viscera and red palm oil, respectively. There was significant difference (p<0.05) among the means of moisture, protein, lipid, PV and IV but the ash content shows no significant difference (p<0.05). The result of the fatty acids composition shows that red palm oil has the highest saturated palmitic acid and monounsaturated oleic acid 44.29 and 40.62%, respectively. The 10.20% palmitic acid and 6.20% oleic acid was observed in catfish oil. 13.26 and 8.59% linoleic acid, 1.40 and 0.40 stearic acid was observed in catfish oil and red palm oil, respectively. Catfish oil shows dominant unsaturated linoleic and oleic acid, saturated palmitic acid and stearic acid. The result shows that catfish oil is rich in polyunsaturated fatty acid (PUFA), which lowers total serum cholesterol level.