Abstract: A set of light traps were used to sample freshwater shrimp and fish from Barito River in Banjarmasin, South Kalimantan. Each of five collapsible traps (same size and shape) and of four different traps (box-shape, wire-stage, bamboo-stage and minnow nets) was assigned with one of LED lamps or incandescent lamps. A total of 231 shrimps and 34 fishes were collected during the 6-night sampling periods of March 2009. The shrimp catch was dominated by the Macrobrachium sp. (99.6%) with size ranges varied from 24-85 mm total length. The fish catch composed of Oxyeoleotris urophthalmus (38.2%), Glossogobius giuris (20.6%), Oxyeoleotris marmorata (17.6%), Flounder pleuronectes (8.8%), Puntioplites bulu (5.9%), Mastacembelus erythrotaenia (5.9%) and Osteochilus melanopleura (2.9%) with size ranges varied from 60-310 mm total length. It can be concluded that color or relative intensity of light had strong effects on number of catch collected, where LED traps appear to be more effective than incandescent ones. The Minnow nets collected significantly number of catch compared to other three traps regardless white LED lamps and ordinary incandescent ones. The effect of size and shape of the traps that influenced number of catches was further discussed in this study.