Abstract: This study was conducted in order to characterize the different levels of dietary vitamin C and vitamin E on some of hematological and biochemical parameters of sterlet. For this purpose 270 sterlet (Acipenser ruthenus) were divided into 18 groups. Three levels of vitamin E (0, 100 and 400 mg kg-1 diet) and vitamin C (0, 100 and 400 mg kg-1 diet) and their combination were used to prepare nine experimental diets. Each of nine experimental diets was fed to fish in 2 tanks (2 replicates). The fish were fed 3% of their wet b.wt. per day for a 100 days period. Blood samples were obtained from three fish of each tank at the end of experiment. The results reveal that Fish fed diets containing 100 mg kg-1 vitamin E and 400 mg kg-1 vitamin C (diet 7) had the highest WBC (p>0.05). Also, significantly higher RBC was observed in diets 3, 4, 5, 6 and 9 than those of the other diets in which different levels of each vitamin without any regulation exists. The hematocrit percentage did not differ significantly in fish fed the different diets (p<0.05). Also, there was no significant difference in the mean amount of total protein, cortisol, glucose and triglyceride between the fish fed with the different diets designed for this experiment (p>0.05). On the other hand, fishes fed diets without vitamin C but different levels of vitamin E (diets 3 and 6) had significantly higher amounts of cholesterol compared with fish fed with other diets.