Abstract: The composition of lactic acid bacteria (LAB) in intestine of two species of sturgeon, beluga (Huso huso) and Persian sturgeon (Acipenser persicus), was analyzed. LAB in the gastrointestinal (GI) tract of the two sturgeon species was not similar as LAB population levels in beluga was significantly higher than Persian sturgeon. Six strains of lactic acid bacteria isolated from the GI tract of beluga and Persian sturgeon were characterised by 16S rDNA. Two species of LAB including Enterococcus seriolicida and Leuconostoc mesenteroides were isolated from GI tract of Persian sturgeon and the predominant species was L. mesenteroide. Furthermore, Lactobacillus curvatus, Lactococcus raffinolactis, Lactococcus lactis and Streptococcus sp. were isolated from the GI tract of beluga and the counts of L. curvatus was significantly higher in the GI tract of beluga than other species.