Abstract: The present investigation deals with the distributions of three heavy metals, lead (Pb), cadmium (Cd) and chromium (Cr) in sediment and their availability in tissues of Labeo rohita (Hamilton) fingerlings. This study showed that Cd was available in exchangeable and carbonate fractions of the sediment, where as Pb and Cr was mainly existed in organic and residual organic fractions. This study revealed that Pb and Cr were more in gill tissues, while Cd was mainly concentrated in viscera. Significant relationships (p<0.05) between metals in different chemical fractions (exchangeable, carbonate, Fe-Mn oxide, organic and residual) of sediment and metals in fish organs (Pb in all organs, Cd in muscle, skin and viscera and Cr in muscle and gill) were found.