Abstract: A seven week feeding experiment was conducted to evaluate the suitability of incorporation of slaughter house waste in the diet of rohu fish. Fingerlings of Labeo rohita (weight 1.83±0.02 g) were used as experimental animal. Five nearly isocaloric (3.6-3.7 kcal/g) diets with 23.96 (control), 25, 30, 35 and 40% protein level were formulated. Slaughter house waste in the diets was incorporated at levels of 14.62, 24.32, 34.04 and 43.75% to replace equal proportion of the other ingredients. Fish were fed twice daily with the respective test diets at the rate of 4% body weight during entire culture period. Results regarding growth performance and feed conversion of Labeo rohita fingerlings fed different experimental diets showed that the best growth performance of fish in terms of percentage live weight gain (153.27%) was found with Diet 2 having 30% protein level and lowest (96.26%) found with control diet. Specific Growth Rate (SGR) of fish fed different levels of protein showed that highest (2.48) SGR was found with 30% protein feed and lowest (1.55) SGR was found in control diet. Significantly (p<0.01) higher SGR was found in diets having slaughter house waste, compared to control diet. The feed conversion efficiency (52.72) was highest in fish fed on Diet 2 compared to all other diet. Feed conversion efficiency was significantly (p<0.01) higher in fish fed on slaughter house waste based diet compared to control diet. Survival rates were recorded highest in fish reared with D2 and D3 diet (100%). Analysis of carcass composition of fish fed with various diets also showed that the fish fed with D2 was rich in their body carcass protein and fat content (58.34 and 13.21, respectively), whereas they were low in their moisture content compared to other experimental diets. This study brings home to fact that slaughter house waste is one of the best animal proteins in terms of growth, feed conversion and body carcass composition and 30% protein level is optimum for growth of Labeo rohita.