Abstract: Background and Objective: Biogas production from raw material is a renewable and clean source of energy. This research work investigate the biogas production from poultry droppings mixed with plantain peels and poultry droppings without plantain peels. Materials and Methods: Chemical and proximate compositions of digestate were carried out with the aids to determine the potential use for agricultural purposes. Statistical analysis was performed using Microsoft Excel and 4 factor Response Surface Methodology (RSM). Results: Result obtained from poultry droppings mixed with plantain peels and poultry droppings without mixing with plantain peels indicates the maximum biogas yield. The predicted values were validated and the average optimum biogas volumes obtained. Analysis of variance of regression equation shows the coefficient of determination (R2) of 99.67 and 99.98%, respectively. The measured temperatures (ambient, slurry, gas layer, interface between slurry and gas layer) during the fermentation process were found to be within the mesophilic temperature range. The chemical and proximate compositions of the digestates show that it could be used as replacement for biological fertilizers. Conclusion: Therefore, it can be concluded that mixing plantain peels with poultry droppings increased the biogas volume.