Abstract: Background and Objectives: Vermicompost known as one of the natural organic fertilizers, which were commonly available to plant and environmental friendly. The objectives of this study was to produce vermicompost from agricultural waste by using Eudrilus eugeniae (E. eugeniae). The qualities of vermicompost and biomass of E. eugeniae were also investigated. Materials and Methods: About 100 g of E. eugeniae were cultivated in plastic containers (40×40 cm) with 2 kg of various types of agricultural waste. After 60 days of cultivation, biomass of E. eugeniae and nutrient contents in vermicompost were determined. Results: Vermicomposts produced by E. eugeniae had high major nutrient contents consisting of 1.108% nitrogen, 0.669% phosphorus and 1.318% potassium. Specially, all treatments showed major nutrient values of vermicomposts which were higher than those of standard organic fertilizers. Furthermore, E. eugeniae had the overall biomass after 60 days of cultivation, which rose to 28.33-104.67% compared to its initial weight. Conclusion: The data provided new evidence that agricultural waste, especially soybean meal could be used as feeds for the high quality of vermicompost production and earthworm biomass. Thus, vermicompost producing from agricultural waste was a new source of nutrients in addition to organic compost and chemical fertilizers.