Abstract: Background and Objective: Beta-cyfluthrin, one of the most widely used pyrethroid insecticides against broad-spectrum insects in agriculture and in public health sectors. In domestic and agriculture uses, humans in all life stages e.g., adult (men and woman), pregnant women, newborns and children may have suffered potential exposure to β-cyfluthrin. Therefore, the present study was conducted to evaluate the adverse effects of 60 days sub-chronic exposure to β-cyfluthrin on male rats. Methodology: Twenty four male rats were assigned to four groups, control group and β-cyfluthrin-treated groups. Rats of β-cyfluthrin-treated groups were orally administrated β-cyfluthrin at doses 15.2 mg kg–1 b.wt. (1/25 LD50), 7.6 mg kg–1 b.wt. (1/50 LD50) and 3.8 mg kg–1 b.wt. (1/100 LD50) in corn oil, daily for 60 days, respectively. Blood hematological parameters, (Red Blood Cells (RBC), White Blood Cells (WBCs), hemoglobin (Hb), hematocrit (PCV) and platelets (PLT)) serum liver and kidney biomarkers (aspartate aminotransferases (AST), alanine aminotransferases (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), protein, albumin, uric acid and creatinine) and testosterone hormone were determined, in addition to histopathological investigations. Results: Results showed that β-cyfluthrin caused significant reduction in body weights (b.wt.) and increase in relative liver weights at high and medium doses. It caused significant decrease in RBC’s and PLT count, Hb level, HCT volume and PCV% and significant increase in WBC’s count. Significant increase in AST, ALT, ALP, LDH, globulin, protein uric acid and creatinine, while decrease in albumin and testosterone hormone were recorded in β-cyfluthrin-treated rats in dose dependent manner. It induced histopathological alteration in liver, kidney and testes tissues. Conclusion: In light of the results in the present study, it can deduce that β-cyfluthrin induced liver, kidney and testes damage in male rats. Therefore, some precautions must take mainly among agriculture workers especially in poor rural where workers and their families (woman, pregnant women, newborns and children) are working and housing close to area of pesticides application and storage.