Abstract: The textile industries produces considerably high amount of aquatic toxicity which is discharged directly into the environment before treated properly. The waste generation volume and load produced is hazardous in nature. Thus, this study explores the role of fungal biomass against pollution due to textiles dyes as degrading agent. This study will be beneficial for treating water effluent from textile industry and will decrease the pollution form environment with advanced technology for future use. In this study the evaluation of fungal species for the decolourization and degradation of textile dye has been carried. Four potential fungal strains (NS-1, NS-2, NS-9 and NS-10) were exploited after screening for the decolourization of Rubine Toner-12 dye under aerobic condition. Growth associated decolorization studies were carried out in Potato Dextrose Broth (PDB) supplemented with Rubine Toner-12. About 99% percent decolorization was achieved on supplementation with 10 mg L-1 of dye. Comparative spectrophotometric analysis of control and fungus inoculated medium supplemented with rubine toner-12 showed almost 100% decolorization in inoculated flasks. The fungus was identified to be Aspergillus niger. Maximum decolorization of Rubine Toner-12 was observed at pH 6. It is a better technique to check environmental pollution.