Abstract: Background: Annona mucosa Jacq. is a promising species for pest control due to its effectiveness against some insects. The insecticidal effects of crude extracts of A. mucosa seeds on the soybean looper, Chrysodeixis includens (Walker) an insect pest of several crops was evaluated. Materials and Methods: The extract was prepared and diluted in water with solubilizer at the concentrations of 0.5, 1.0, 2.0, 4.0 and 8.0% and applied topically and orally to first, third and fifth instar larvae. Results: The extract of A. mucosa exhibited insecticidal properties against all instars of C. includens. Mortality of first instars was high after 24 h for both application methods. No differences were observed in mortality of third instar larvae exposed by ingestion of leaves treated with the extract at different concentrations. Mortality reached 80% of third instar larvae 24 h after exposure by contact with the highest extract concentration. Among fifth-instar caterpillars fed on leaves treated with the extract, mortality was significant only after 72 h at the concentrations between 2.0 and 8.0%. Topical application of A. mucosa extract resulted in mortality of 93.3% at the highest concentration after 24 h of treatment. Conclusion: The results show that A. mucosa is a promising species for the development of new toxic molecules for the control of C. includens.