Abstract: Uneven distribution of food resources to offspring by females plays an important role in adaptation to environmental changes in many taxa. In the honeybee, the differential maternal investment has a potential role in the amplification of intra-colony phenotypic variability which is an important factor in stress resilience. In this study, the repeatability of weight measurements and the optimum stage was determined when the eggs were sampled to minimize the imprecision due to unavoidable intra-sample age differences. Eggs were weighed eggs from fourteen selected colonies and assessed the relative weight variability. A comparison was done between the means and variability of eggs produced in spring and late summer were compared to test the hypothesis that eggs destined to become a mix of summer and winter bees those should be more variable than eggs all destined to become summer bees. The results showed that the optimum age for sampling eggs is 48 h. No systematic difference was found between spring and summer samples but the difference in sample means from the same queens was up to 22%. Whereas, the difference in weight of eggs laid by the same queen within six hours was up to 58%. A comparison with published data on the effects of difference in egg weight from reared adults showed that the level of variability observed was sufficient to expect phenotypic differences at the adult stage. Therefore, it can be concluded that a considerable level of differential maternal investment exists in the honey bee. The research findings provided an opportunity for further studying its consequences and evolutionary significance in social species.