Abstract: This study was carried out to assess the biological activity of methanolic extracts and fractions from Ipomoea murucoides [Roem et Schult (Convolvulaceae)] on Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). The extracts were incorporated into a meridic diet in 2 mg mL-1 and fed to 1st instars larvae of S. frugiperda and incubated at 27°C with a photoperiod of 16:8 (L:D) h. After 7 days, surviving larvae were counted and weighed; surviving pupae were incubated until moths emerged, sexed and females allowed to lay eggs on paper foil. Fecundity was measured. The crude leaf extracts produced up to 46.16% mortality with effects on development, reduction in larval weight to 42.26 mg in 3rd instar and 59.6% in 5th instar, increased time for pupation and in reaching the adult stage; there was no effect on number eggs laid. The LC50 calculated for methanolic leaf extract had a value of 2.692 mg mL-1. The partially purified fractions showed no toxicity toward S. frugiperda, but had the highest effect on larval weight reduction, to 76.3% in 3rd instar and 74.6% in 5th instar, increased time for pupation and time to reach adult stage and had an effect on number eggs laid. These results indicate that the tested compounds delayed larval development.