Abstract: Background and Objective: Looking at the present world scenario, sustainable development and environment-friendly living have become the most significant focus. In this study, the efficiency of watermelon seeds in the production of biodiesel was tested. Furthermore, watermelon seeds are evaluated to see if they can be used as a feedstock for the production of biodiesel. Materials and Methods: Watermelon oil was made into biodiesel through transesterification and evaluated the properties of the biodiesel like flash point, fire point, viscosity, calorific value and specific gravity. Various blends of biodiesel, i.e., B10, B20 and B30, were prepare and measured the properties of these blends. The above mixtures were used in a diesel engine, carried out performance tests and emissions tests and compared these results with petro-diesel. Results: This study identified the best blend that can be used as a working fuel. As per the blends prepared (B10, B20, B30), B10 proved more efficient in starting the machine. B10 had the highest amount of diesel and less viscosity. It had a minimum viscosity, among others. B10 has the minimum value of the flashpoint and fire point. B10 had the entire characteristics similar to all diesels. Conclusion: The best blend was identified as B10 in terms of performance and can be used as a potential feedstock for the production of biodiesel.