Abstract: Background and Objective: Solanum genus (Solanaceae) is well known by a diversity of active phytochemicals with multi disciplinary biological applications. The study aimed to evaluate the activity of methanolic extract and isolated glycoalkaloids of Solanum torvum callus as antimicrobial in vitro and also to evaluate their cytotoxic role against human carcinomas cell lines. Materials and Methods: Callus cultures of S. torvum were established on Murashige and Skoog media with plant growth regulators. The main constituents of the methanolic extract were identified using HPLC analysis. Anticandidiasis activity was examined against candida albicans. The antibacterial performance was checked versus Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeroginosa. Cytotoxic activities were performed contra melanoma, breast and lymphoma cell lines. Results: Present outputs illustrated that callus cultures were successfully established and accomplished with biosynthesis of the distinguished solanum glycoalkaloids (15.18%). Phenolics were also identified in the in vitro cultures as flavonoids (9.80%) and coumarins (3.41%). Regarding anti candidiasis, current results demonstrated high activities of both S. torvum callus extract and isolated glycoalkaloids with distinctive effect of the later comparing to nystatin. Present data also, illustrated that glycoalkaloids were more active against Gram +ve bacteria while callus extract was more active against Gram -ve bacteria. Plant materials exhibited promising cytotoxic activity against the examined carcinomas cell. Total callus extract showed higher cytotoxic activity than isolated glycoalkaloids which might return to synergistic effect of other phytochemicals. Conclusion: S. torvum cultures are promising routes for developing biologically important active compounds that could be applied as favorable antimicrobial and cytotoxic agents.