Abstract: Background and Objective: Invertebrates comprises more than fifty percent of marine animals. Among them, sponges are the most prolific source of bioactive compounds. Suberites aurantiacus (S. aurantiacus) is an abundant sponge in Mexican Pacific, which has been scarcely studied, for this reason authors screened it for its bioactivities. The current study proposed to determine the enzymatic inhibition and scavenger, toxic and antibacterial activities from Suberites aurantiacus. Materials and Methods: S. aurantiacus samples were collected in Magdalena Bay, Mexico. The ethanolic extract and its fractions were assayed for their antioxidant effect using DPPH, ABTS and NO assays, evaluated their toxicity against Artemia salina (A. salina) and their antibacterial activity against Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), Enterococcus faecalis (E. faecalis), Enterobacter aerogenes (E. aerogenes), Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) as well as their inhibitory effect on α-glucosidase and α-amylase. Results: The results showed median lethal doses (LD50) against A. salina < 1 mg mL–1 for two fractions and a moderate antibacterial activity against Gram positive bacteria. Conclusion: In view of these results, S. aurantiacus could be considered a potential source of antibacterial compounds.