Abstract: Background and Objective: Antimicrobial susceptibility testing of bacteria recovered in diabetic wounds remains an important way of monitoring infection and the emergence of antibiotic-resistant bacteria. Early diagnosis of microbial resistance patterns is aimed to help institute the appropriate antibacterial therapy and improve the adverse effects of diabetic wound infection. This study aimed to determine the phenotypic resistance patterns of the bacteria isolated from the wounds of hospitalized diabetic patients. Materials and Methods: Antimicrobial disk diffusion assay was performed on 42 bacteria isolates using antibiotics of different classes. Variants were analysed using one-way ANOVA and two-way ANOVA through Graphpad prism version 6. Results: Multidrug resistant patterns were noted among different bacterial groups such as Enterococci (83%), Enterobacteriaceae (55%), Non-Enterobacteriaceae (50%), Staphylococci (43%) and Gram-positive rods (33%). The bacteria conferred resistance to penicillin (100%), ampicillin (91%), cefepime (60%), ceftazidime (55%) and gentamicin (52%). Hospital X’s bacteria were found to be most resistant to erythromycin (80%) and ciprofloxacin (70%), while hospital Z’s bacteria were most resistant to vancomycin (50%) and penicillin (50%), with Hospital Y’s bacteria showing the most resistance to Imipenem (45%). Proteus mirabilis showed 86% resistance to Imipenem while Klebsiella spp. and Escherichia coli also exhibited resistance to important antibiotics. Conclusion: The noted high levels of antibiotic resistance and multi-drug resistance patterns, observed in the study are of grave concern as it limits treatment options thereby negatively impacting on the health and quality of life of the affected diabetic patients. It is therefore, imperative that these findings be taken into consideration during public health policy making and awareness programmes.