Abstract: Background: Moringa oleifera extract is mainly used in chicken as a feed additive but their effect as water supplementation on performance, carcass characteristics, immune response and blood antioxidant level were rarely studied. Material and Methods: Different broiler breeds were studied using 120 Cobb 500 chicks and 120 Ross 308 chicks which were distributed into 3 treatments (40 birds treatment–1) including birds drink water supplied with 2% Moringa oleifera leaves aqueous extract (MW 2%), water supplementation with 3% Moringa oleifera leaves aqueous extract (MW 3%) and water without any supplementation (control); moreover chicks of each treatment were distributed into 4 replicates (10 birds replicate–1) from 2-6 weeks. Results: Moringa oleifera 3% treated chicken recorded significantly higher WG2 and WG3 than Moringa Oleifera 2% however chickens at control groups had significantly worst FCR1 compared to Moringa Oleifera treatments, moreover; the response of both breeds to the higher concentration of Moringa oleifera was better. Ross breed groups achieved better results than Cobb breed in performance, total Lactobacillus count and immunity against newcastle disease virus vaccine. There were no significant differences between treatments in carcass characteristics and blood total antioxidant capacity. Conclusion: The researchers found that benefits from Moringa oleifera leaves water supplementation may be obtained at high concentration with better response for Ross over Cobb breed. Many studies should be applied on application of Moringa oleifera in drinking water.