Abstract: Background and Objective: The routine technique applied in spawning of sea bream (Sparus aurata), in the hatchery depends on the use of a mixture of synthetic luteinizing hormone releasing hormone (LHRH-a)+human chorionic gonadotropin hormone (HCG). The present study aims to use HCG alone as a method to take the advantages of HCG specifically lower production costs. Materials and Methods: Adult and fully ripe Sparus aurata were injected with either one of two hormone protocols using two hormones, human chorionic gonadotropin hormone (HCG) and a mixture of luteinizing hormone releasing hormone analogue and HCG (LHRH-a+HCG) during the spawning season. The two hormone protocols were applied through a single dose of each hormone. The first protocol was applied by 5000 IU HCG kg–1 and the second protocol was applied by using 5000 IU HCG+200 μg LHRH-a kg–1. A comparison between the two protocols is demonstrated in the present study. Forty four ripe fish (16♀+28♂) were divided into 2 groups each of (8♀+14♂) and were injected intramuscularly with a single dose of human chorionic gonadotropin hormone (HCG 5000 IU kg–1) or mixture of luteinizing hormone releasing hormone [LHRH-a (200 μg kg–1)+HCG (5000 IU kg–1)]. Data were analyzed using overall one-way analysis of variance (ANOVA) and differences were observed at significance of p<0.05. Results: In the females of the two experimental groups, spawning was induced in 54 and 48 h after injection in the mixture and the single hormone protocol, respectively. A comparison between both protocols showed that both treatments were found to be equipotent in inducing fish spawning with superiority of LHRH-a+HCG mixture over HCG. Fertilization and hatchability rates as well as eggs and larval quality recorded increased values in group injected with hormones mixture. Although no histological differences were seen in pituitary section of the 2 groups, the number of gonadotrophic cells and vacuoles diameter/unit area showed differences between the two groups. The correlation value and F-test showed that there are not any significant differences between the values of survival percentages, average length and average weight in cases of HCG alone or LHRH-a+HCG. Conclusion: Even though the superiority of LHRH-a+HCG mixture over HCG but for economical purpose, the use of HCG alone is preferred.