Abstract: Background and Objective: In developing countries such as Iraq, every year there are thousands peoples infected with typhoid fever caused by Salmonella typhi. These infections became difficult to treat by different types of antimicrobials. Therefore, the main aim of this study was to investigate the ability of S. typhi strains isolated from blood of inpatients and outpatients infected with typhoid fever to produce some antimicrobial resistance associated-genes. Materials and Methods: Disc diffusion method was used to investigate the ability of S. typhi to resistance of 12 antibiotics and PCR technique were used to investigate the prevalence of 13 antimicrobials resistance genes. Fisher's exact test was used for the comparison between samples of the study and then analyzed with graph pad prism version 5. Results: The results proved that 61.53 and 51.28% of S. typhi strains were resistant to ampicillin and chloramphenicol, respectively and all strains (100%) were susceptible to ceftriaxone 30 μg. Among 13 antimicrobial resistance genes, the most prevalent was floR (74.35%), while there was no prevalence of blaCMY-2, Cat3 and strA-strB genes. Conclusion: The floR, Cat1 and pse-1 genes were the most prevalent in S. typhi strains isolated from blood of inpatients with typhoid fever more than those isolated from blood of outpatients.