Abstract: Background and Objective: Iridaceae is a widespread family which include 92 genus and 6 of them are spreading in Turkey. This family includes wild plants whose endemic species have attractive flowers. Iris sari is a folk medicinal plant in Southeastern of Turkey. This study aimed to investigate the biological and bioactive properties of I. sari in Turkey. Materials and Methods: In this study, n-hexane, dicloromethane, methanol and water extracts from different parts (flower, leaf and rhizome) of I. sari were obtained by Soxhlet apparatus and their antioxidant, antibacterial and DNA protection activities were studied. Antioxidant activity, total antioxidant and oxidant levels were determined by using Relassay Diagnostics Kit (Antioxidant Kit, Turkey) and DPPH assay was used to determine antibacterial activity. Microdilution assay was conducted by using 9 different bacterial strains (S. aureus ATCC 25923, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 700603, S. aureus ATCC 29213, E. coli ATCC 25322, E. coli ATCC 35218, E. coli ATCC 10799, E. coli ATCC 8739 and S. aureus ATCC 6538) according to CSL. To determine DNA protection activity, pBR322 plasmid DNA assay were used. Results: Results showed that water and methanol extracts of I. sari showed high antioxidant activities in both antioxidant methods. Although the highest antioxidant and antibacterial activities were found in root water extract, the most DNA protection effect was found in water and methanol extracts isolated from leaf and flower. Conclusion: It was concluded that the endemic and medicinal plant; I. sari contain active metabolites which are responsible for these antioxidant, antibacterial and DNA protection activities.