Abstract: Background and Objective: Biofilm plays an important role in causing microbial corrosion. One of prerequisites for microbes as energy source to grow and to form biofilm is carbon source. The purposes of this study were measuring the ability of biofilm community sampled from Saguling hydro power in utilizing carbon sources and assessing culturable heterotrophic of bacterial community from those biofilm. Materials and Methods: Biolog EcoPlateTM and culture-dependent approach were used to assess biofilm community. Results: Heterotrophic bacteria in biofilm have the ability to use 30 different carbon sources consistently. The source of carbon used by this community at the highest rate are N-acetyl-D-glucosamine from carbohydrates group, 2-hydroxybenzoic acid from carboxylic and kenotic acids, glycogen from polymers group and L-serine from the amino acid group at the rate of 0.16, 0.05, 0.14 and 0.09 absorbance U h–1 respectively. Whereas the carbon sources with high consumption are as follow: Pyruvic acid methyl ester, β-methyl-D-glucoside, D-mannitol and N-acetyl-D-glucosamine from carbohydrates group; D-galacturonic acid, 2-hydroxybenzoic acid, D-glucosaminic acid and D-malice acid from the group of carboxylic and kenotic acids; tween 40 and 80 as polymers; L-argentine, L-asparagines, L-serine, L-heroine and glycol-L-glutei from amino acids group. Furthermore, culturable bacterial community of those biofilm were dominated by Gram-negative bacteria, consisted of five common/heterotrophic bacteria, two manganese bacteria, two nitrifying bacteria, three iron bacteria and three sulfate reducing bacteria. Conclusion: Bacterial community of biofilm from Saguling hydro power which were dominated by Gram-negative bacteria have the ability to use various carbon sources and degrade glycogen.