Abstract: To determine essential oil composition, antioxidant and antimicrobial features of the aerial parts of Chaerophyllum crinitum Boiss. The obtained oil of the plant analysed by GC and GC/MS. The analysis has led to the identification of 64 components comprising 85.5% of the oil. In addition, DPPH assay and disc diffusion method were used to evaluate antioxidant and antimicrobial properties, respectively. α-terpinolene (20.3%), β-cubebene (9.3%), α-terpineol (7.2%) and limonene (5.8%) were dedected as major components. Moreover, C. crinitum exhibited remerkable antimicrobial effect against most of used microorganisms. But it was inactive in the DPPH assay. The chemical composition, antioxidant and antimicrobial effects of the essential oil of aerial parts of C. crinitum were reported for the first time and obtained results show that it may be a good candidate for the future studies.