Abstract: An experiment was conducted at Bako Agricultural Research Centre in the production season of 2011/12 with the purpose of identifying the best combination of inter and intra row spacings for the optimum production of potato (Solanum tuberosum L.) seed tuber with marketable size and good flour quality. Six levels of inter (60, 65, 70, 75, 80 and 85 cm) and three levels of intra row spacings (20, 30 and 40 cm) were used in 6x3 factorial combination arranged in a randomized complete block design with three replications. Most of the observed variables were significantly affected due to the variation in the treatment combination between inter and intra row spacings. Narrow spacings shifted seed tuber distribution from larger to the smaller and undesirable tubers considered from marketable point of view. Though, total yield increased under narrower spacing conditions, marketable tubers are more promising for encouraging farmers to continually produce seed tubers. These therefore require relatively wider spacing to have more number of marketable size tubers. To achieve this, the study identified 70-75 cm inter and 20-30 cm intra row spacing as the best spacing combination for optimum yield and good quality potato seed tuber.