Abstract: The objective of this research was to produce a bio-based nano material from surian (Toona sinensis Roem) wood bark (SWB) using conventional balls milling and four of sieve types (T77, T90, T120 and T200). SWB is an organic material waste from community forest was subjected to conventional balls milling for 96 h and was converted into bio-based nanomaterial. Bio-based nanomaterial was characterized for its particle size, chemical components, functional groups and crystallinity by using Scanning Electron Microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Diffraction (XRD). The particle size obtained from SEM analysis were found to be 93-110, 67-165, 68-93 and 42-114 nm produced from T77, T90, T120 and T200 of sieve types, respectively. The nanoparticle of organic material from SWB was obtained with the size in diameter was less than 1000 nm. The proportion of nano particle size were 39, 29, 24 and 2% produced from T77, T90, T120 and T200 of sieve types respectively and the last (6%) of nano particle retained on T77 of sieve type. The chemical components found in bio-based nano material from SWB obtained from EDS analysis were carbon, oxygen, potassium and calcium. The FTIR analysis shows the presence of C = O stretching band at 1735 cm-1 in the nanoparticle of SWB produced from T90 and T120 of sieve types, respectively and C = O stretching band at 1734 cm-1 of SWB produced from T200 of sieve type, due to balls milling effect while the nanoparticle of SWB produced from T77 of sieve type not observed. Whereas the crystalline structure was found calcium oxalate hydrate (C2CaO4.H2O) with diameter of crystalline size were 26 and 14 nm produced from T77 and T200 of sieve types, respectively.