Abstract: Mushroom considered as an important food it has healthy and economic benefits. This study was aimed at investigating the medical effects of edible mushroom extracts on aflatoxin B1 for the kidney, liver and SOD enzymes. Thirty-six male Sprague-Dawley rats weighing 120-150 g were divided into six groups (6 rats/ group) and housed in stainless steel cages housed in a temperature-controlled (23±1°C) and artificially illuminated (12 h dark/light cycle) room free from any source of chemical contamination. Fine powder of mushroom was suspended in gum acacia (2.5% v/v in distilled water) into two dose levels (100 mg and 200 mg/1 mL/kg body weight). The 1st group kept as a control group; the 2nd and 3rd groups were given 100 mg and 200 mg kg-1, mushroom, respectively, the 4th group treated orally with aflatoxin B1 2.5 mg kg-1, in corn oil, the 5th and 6th groups administered 100 mg and 200 mg kg-1 mushroom, respectively in concomitant with AFB1. The experimental period was 15 days of daily treatment after which blood samples were collected from the retro-orbital vein of each animal, under light anesthesia by diethyl ether. Serum obtained from each animal was used to estimate the activities of Aspartate amino-transferase, Alanine amino-transferase, Superoxide dismutase, Creatinine and Uric acid. The results indicated that a low dosage of mushroom 100 mg with aflatoxin B1 was the best treatment to decrease the toxicity in animals significantly (p<0.5) and these clearly demonstrated that, mushroom was a good functional food because it has many medicinal compounds.