Abstract: This study analyzed the effect of various Edaphic factors on the Vesicular Arbuscular Mycorrhiza (VAM) associated with medicinal plants. Based on the richness of medicinal plants in Himalayan region, this study considered three plants i.e., Catharanthus roseus Linn., Ocimum spp. and Asparagus racemosus Willd. The study was conducted at five districts of Uttarakhand state in India viz. Pauri Garhwal, Haridwar, Dehradun, Udham Singh Nagar and Almora. This study has evaluated and analyzed the effect of edaphic factors like pH, organic carbon, potassium, phosphorous and soil type on the spore number. The correlation between the individual variations of different edaphic factors with spore number was also investigated. Change in edaphic factors greatly influence mycorrhizal diversity. Maximum number of spores were isolated from soils of pH 6.0-7.0 and with an organic carbon contents ranging from 0.82 to 0.85%. Generally, phosphorous content in the soil samples ranged from 27.11 to 37.21 k ha-1 and a feeble negative correlation was observed with respect to spore population. As the moisture content of the soil increased, it was observed that VAM fungal spore number decreased. Potassium content of soil showed significant positive correlation with spore numbers. Glomus species was observed as more versatile in comparison to others and therefore existed in even adverse soil and climatic conditions.