Abstract: Antioxidant potential of Eugenia jambolana seeds was carried out using various in vitro models. Ethanolic (50%) extract at 1000 μg mL-1 showed maximum scavenging of the radical cation, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) observed upto 98.92% followed by scavenging of nitric oxide radical (96.75%), ferric ion radical (94.43%), 1,1- diphenyl, 2-picryl hydrazyl (DPPH) (92.25%) and antilipid peroxidation potential (81.13%). The antioxidant activity of the seeds, help in preventing the oxidative damages caused and also possess α glucosidase inhibitory activity, which are very vital in the management of Diabetes mellitus. The finding justifies the therapeutic application of the plant in the indigenous system of medicine, augmenting its therapeutic value.