Abstract: In the present research, we decided to study about the protective effect of vitamin E against Azathiprine-induced toxicity. In this study, 40 male Wistar rats were divided to 4 groups (each group contains 10 rats). For the first group, as the control one, normal saline was given. The second and third groups received 20 mg kg-1 of vitamin E daily and for 7 days by Intra Muscular (IM) injection. The forth group, that had similar state with three others, normal saline was injected for 7 days. On the seventh day, both 3 and 4 group treated by 15 mg kg-1 Azathioprine as a single dose and Intra Peritoneal (IP) form. Two other groups only received the dissolvent of Azathioprine in the same dose and manner. Twenty four hours after Azathioprine injection, the animals after being weighted were anesthetized by ether and blood sample were taken via., the tail vein and pathological sample was got from liver. The samples were allowed to clot and then their serum was separated by centrifuge machine of 2500 rpm for 10 min. This study showed that Azathioprine-induced damage on liver in group 3 is less than group 4 and function of organ in group 3 is nearly same with control group. Results of this study demonstrated that vitamin E decrease Azathioprine-induced hepatotoxicity in rat. According to surveys that have done, the necrotic regions and hepatic cellular death in liver was so lesser in the group that treated together with Azathioprine and vitamin E than the group that treated only by Azathioprine and was so similar to control group. Generally, the pathological results of this study confirm the biochemical results.