Abstract: The infection by cyst-producing protozoa (Toxoplasmosis, Sarcosporediosis and Neosporosis) in human and animals in Egypt was investigated using EITB and micro ELISA technique. Two specific eluted and concentrated protein fractions were used as antigen. The molecular weight (MW) of these fractions is 32 kD for Toxoplasma gondii tachyzoites Ag. (TTAg) and that corresponding to 53 kD for Sarcocystis bradyzoites Ag. (SBAg). The data cleared a direct relation between contact with animals and the increase in the rate of infection by these parasites among human. The incidence in female and male who work in continuous contact with animals was 36 and 24%, respectively. It was 17 and 19% in females and male patients who are in in-direct contact with animals and visit hospitals complained by mayligia, arthritis and gastro-intestinal disturbances. The low incidence was recorded in non-complained youth (1% in females and 3% in males). The mean rate of infection by the three parasites was high in the examined apparently healthy old buffaloes, cattle and sheep. It was 46.5, 22.5 and 25.6% in the above animals respectively. Sarcosprodiosis is the most common one followed by toxoplasmosis, while the infection by Neospora is the lowest one. Toxoplasmosis is high in women`s and female animals. Sarcosprodiosis is high in men`s while no effect for sex in animals. High sensitivity and specificity of these fractions will improve the diagnostic utilities of these parasites, the matter which improves capability of the related ministries to minimize their distribution in Egypt.