Abstract: The aim of present study was to investigate the effect of hydroalcoholic extract of Ruta chalepensis on the ileum contractions by some known spasmogens and also to study the possible mechanism involved. Rue extract was prepared by macerated with alcohol (70%). A piece of ileum (2 cm) was removed from male Wistar rats and mounted in an organ bath containing air bubbled Tyrode solution with 1 g initial tension and contractions were recorded by an isotonic transducer. The relaxed ileum by non-cumulative concentration of Rue extract (0.01, 0.02, 0.03, 0.04 mg mL-1) were effected on KCl (60 mM) and ACh (1 μM) spasmogens. The results demonstrate that the Rue leaf extract significantly dose-dependently (p< 0.001) reduces the ileum contractions induced by KCl (60 mM). Acetylcholine (1 μM)-induced ileum contractions were attenuated at the same concentration of the extract (p< 0.0001). The Rue leaf extract (1 mg mL-1) induced relaxation on the ACh-induced contraction in ileum was unaffected by beta-adrenoceptor antagonist (propranolol 1 μM). Cumulative concentrations of extract (0.01, 0.02, 0.03, 0.04, 0.05, 0.06 and 0.07 mg mL-1) have reduced the KCl-induced ileum contractions in a dose dependent manner and significantly (p< 0.001). The spasmolytic effect of the cumulative concentration of extracts (0.01, 0.02, 0.03, 0.04, 0.05, 0.06 and 0.07 mg mL-1) was reduced after tissue incubation 30 min with propranolol (1 μM). Furthermore, it seems the portion relaxtory effect of Rue extract on the rat ileum may be due to β-adrenergic receptors.