Abstract: Virulence of three indigenous Iranian isolates of the fungus, Metarhizium anisopliae (Metsch.) Sorokin (Deuteromycotina: Hyphomycetes), named DEMI001, IRAN 715C and IRAN 1018C, were evaluated as well as their virulence and their ability to suppress populations of the granary weevil, Sitophilus granarius L. (Coleoptera: Curculionidae). Five aqueous suspensions were prepared from each isolates, in a logarithmic series in Tween 80 (0.05% v/v). LT50 values ranged from 5.54 to 7.9 days following immersion in aqueous suspensions. Lowest LC50 on day 10 was 1/4x105 conidia mL-1 for DEMI001. Cumulative mortality 10 days after treatment varied from 9.4 to 88.88% for IRAN 1018C at low and high concentration, respectively.