Abstract: In view to valorize the traditional medicine of Togo, a comparative phytochemical screening was undertaken on the leaves powder extracts of Annona senegalensis of Togo and the one originates from Burkina Faso. The different extracts were obtained by splitting (percolation) and by steeping the vegetable material with appropriate solvents. The main chemical groups of the extracts were characterized by the aid of specific chemical reactions. The 70% aqueous methanol and aqueous extracts were analysed using High-Performance Liquid Chromatography (HPLC). The rate of the relative humidity in Togolese leaves powder was higher (9.7 ± 0.9%) compared to the one from Burkina Faso (7.0 ± 1.1%) (p<0.05). The chemical tests in the limit of their sensitivity and their specificity allowed us to detect some various components hereafter: sterols/triterpenes, carotenoids, flavonoids, anthocyanosides, saponosides and tannins. The HPLC assay has confirmed the presence of some flavonoids, namely rutin and isoquercetrin (specimen from Togo) and the preceding compounds plus epicatechin and catechin derivatives (specimen from Burkina). These differences in the qualitative composition of bioactive compounds of extracts corroborate with the role of the ecosystem on the phytochemical constituents of the two species.