Abstract: In this study, methanolic extracts of 14 traditional plants in Malaysia were screened for insulinotrophic properties, using rat pancreatic β-cell lines, BRIN-BD11 cells. In 30 min acute static incubation test, all 14 plants showed varying degree of responsiveness in insulin release with Labisa pumila, Morinda citrifolia, Momordica charantia and Tinospora crispa having high insulinotrophic activities. These plants also displayed appreciably low cytotoxic activities. These results show a promising avenue for development of novel insulin secretagogues.