Abstract: A monocot mannose-binding Amaryllidaceae lectin from the bulbs of Crinum latifolium, specific for methyl-α-D-mannopyranoside, has earlier been reported as a nonmitogenic, nonglycosylated homodimeric protein with a subunit molecular mass of 12 kDa. In addition to the conventional protein purification techniques, lectin has also been purified using reverse phase high-performance liquid chromatography and evaluated for biological properties such as antiviral and cytotoxic potential. Lectin did not show inhibition against five human cancer cell lines representing different organs and tissues. Interestingly, it was found to be nontoxic towards BSC-1 cells (African green monkey kidney epithelial cells) and exhibited a significant antiviral potential against a strain of vaccinia virus in vitro.