Abstract: The present research aims to study the molecular characterization of a new 60S ribosomal protein L10 (RPL10) from Mucor racemosus PTCC5305 and its coding sequence. A full-length complementary DNA (cDNA) encoding 60S ribosomal protein L10 was isolated from Mucor racemosus cDNA library. The cDNA of 766 base pairs contains a 652 base pairs open reading frame and 5`- and 3`- flanking regions including a polyadenylation sequence. The deduced protein of 216 amino acid residues had an isoelectric point (pI) of 10.16 and a calculated molecular mass of about 24.5 kDa. Sequence analysis of RPL10 revealed 78% identity and 89% similarity with fungus Pyrenophora tritici. Also it showed 73% identity and 84% similarity with Homo sapiens. Phylogenetic tree analysis based on the conserved domain of Ribosomal_L16_L10e super family exhibited that Mucor racemosus RPL10 is closely related to that of Candida glabrata.