Abstract: Ethanol extract of Capparis aphylla (Roth.) whole plant (EECA) was evaluated for possible testicular antisteroidogenic activity in mature male rat. The ethanol extract at the doses of 50, 100 and 200 mg kgG1 body weight (i.p) arrested the testicular steroidogenisis. The cholesterol and ascorbic acid content in testis were significantly elevated in treated rat. The extract also significantly inhibited the activity of Δ5-3β-hydroxy steroid dehydrogenase (Δ5-3β-HSD) and glucose-6-phosphate dehydrogenase (G-6-PD), the two key enzymes involved in testicular steroidogenesis. Results of this study suggested that the ethanol extract of whole plant of Capparis aphylla (Roth.) acts as an testicular antisteroidogenic agent.