Abstract: Injection of hyperglycemic hormones obtained from crab (Oziotelphusa) and millipede (Spirostreptus) showed different effects on tissue phosphorylase activity and levels of total carbohydrates and glycogen in the crab, Oziotelphusa senex senex. Hyperglycemic hormones obtained from both sources caused significant elevation of hemolymph glucose level in crab in a dose-dependent manner. The crab hyperglycemic hormone induces phosphorolysis of glycogen, whereas the phosphorylase system is not affected by millipede hyperglycemic hormone. The crab hyperglycemic hormone decreased carbohydrate levels in hepatopancreas and muscle, whereas the tissue carbohydrate levels increased after millipede hyperglycemic hormone injection. Administration of millipede hyperglycemic hormone led to significant decrease in tissue lipids, suggesting the involvement of lipids in causation of hyperglycemia. Greater incorporation of labeled acetate-1-14C into the carbohydrate fractions after the injection of millipede hyperglycemic hormone supports the above mode of action. The results demonstrate the existence of at least two parallel mechanisms of hormonal regulation of glycemia in arthropods and point to inter-specific action of arthropod hyperglycemic hormones.