Abstract: All tested Bacillus species in this study had the ability to produce extracellular amylase when grown at pH 5-10 and at incubation temperature between 43-73°C. Bacillus laterosporus and Bacillus schlegelii were able to produce extracellular amylase at incubation temperature of 28°C. Bacillus sphaericus was the only species that produced extracellular amylase at medium pH of 4. Bacillus circulans showed extracellular amylase activity even in the presence of EDTA in its culture media. The presence of NaCl in the culture media variably promoted extracellular amylase abundance with Bacillus schlegelii was able to secrete amylase even in presence of 5% NaCl. The presence of fructose, maltose, lactose and glucose diminished extracellular amylase in all studied species. Presence of detergents Tween-20 or Tween-80 in the culture medium variably affected secretion of amylase.