Abstract: Forty-five animals belonging to four Egyptian goat breeds were undertaken in this study to analyze the different alleles of CSN1S2 gene. The PCR products of five different primers were digested by restriction enzymes MseI, PstI, NcoI, NlaIII and Alw26I for detecting the presence of seven alleles A and B, C, D and 0, E and F, respectively. Present results showed that the homozygous genotypes AA and BB were observed at frequencies 28.9 and 26.7%, respectively and the heterozygous genotype AB was displayed in 18 animals (40%) whereas the genotype AF was present in two animals (4.4%). The alleles C, D, E and 0 were not displayed in tested animals. In conclusion, the improving of Egyptian goat breeds towards economic issues should be directed towards increasing the frequency of homozygous genotypes with the high casein content required for milk industry through selecting animals by molecular analysis.